Team:Panama/Protocols/Wetlab
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- | 1. [[ | + | {{:Team:Panama/template|title=Wetlab}} |
+ | 1. [[Agarose gel electrophoresis]] | ||
- | 2. | + | 2. [[Transformation]] |
- | 3. Colony PCR | + | 3. [[Colony PCR]] |
- | 4. Pgem Cloning | + | 4. [[Pgem Cloning]] |
- | 5. Preparing chemically competent cells | + | 5. [[Preparing chemically competent cells]] |
- | 6. LB media | + | 6. [[LB media recipe]] |
- | 7. | + | 7. [[Ampicillin stock]] |
- | 8. | + | 8.[[Chloramphenicol stock]] |
- | 9. Mutagenesis PCR | + | 9. [[Mutagenesis PCR]] |
- | 10. Mutagenesis transformation | + | 10. [[Mutagenesis transformation]] |
- | 11. Miniprep Quiagen Kit | + | 11. [[Miniprep Quiagen Kit]] |
- | 12. Miniprep "Dirty" | + | 12. [[Miniprep "Dirty"]] |
- | 13. PCR purification | + | 13. [[PCR purification]] |
- | 14. | + | 14. [[Glycerol Stock]] |
- | 15. | + | 15. [[PCR]] |
- | 16. Agar plates | + | 16. [[Agar plates]] |
- | 17. Primer aliquots | + | 17. [[Primer aliquots]] |
- | 18. Cell Culture | + | 18. [[ "Overnight" Cell Culture]] |
+ | |||
+ | 19. [[NZY+ Recipe]] | ||
+ | |||
+ | 20. [[Gel DNA extraction]] | ||
+ | |||
+ | 21. [[SOC Recipe]] | ||
[[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] | [[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] |
Latest revision as of 01:46, 29 September 2011
Wetlab
1. Agarose gel electrophoresis3. Colony PCR
4. Pgem Cloning
5. Preparing chemically competent cells
10. Mutagenesis transformation
12. Miniprep "Dirty"
13. PCR purification
14. Glycerol Stock
15. PCR
16. Agar plates
17. Primer aliquots
19. NZY+ Recipe
21. SOC Recipe