Taking most of the advantages of biofilm as a reactor, Sugarfilm makes phase-related synthesizing procedure (in this case, 3 phases in total) more suitable in biological ways:
(1)Each cell only carries out one reaction step in the system, which greatly relieves stress to the cell when compared with cells carrying the whole process;
(2)The efficiency would not be weakened due to the separation of each step, since the formation of biofilm links all the cells together to get the work done in a nice flow.

What Sugarfilm illustrates here is more than just a simple application. It's a symbol of infinite possibilities coming with our stratified expression biofilm system (Rainbofilm). For similar synthesizing pathways involving a lot of extracellular reaction and intermediate product flow, and relatively clear phase division in the pathway, Sugarfilm would be an ideal model. Only by substituting the corresponding coding sequence parts in the device with the desired coding sequences (enzymes or regulating molecules, etc.) would the system get off to a quick and neat reaction state.

In future development, Sugarfilm should aim to further improve the versatility of the system. First, the targeted substrate of the reactor system should not be limited to saccharide. In fact, Sugarfilm system has the potential to apply to any phase-related synthesizing pathway, as long as the intermediate and final products would not have strong interactions with the biofilm structure (either causing damage to biofilm or be damaged by the extracellular materials within biofilm). Second, to better fit the synthesizing procedure, tweaks about the bacterium strain could be done. Better secretion efficiency and preservation of enzyme function are all vital for whole process. Also, with improvement of the basic Rainbofilm model, especially in accelerating biofilm formation, controlling of biofilm thickness and density, and choosing promoters of higher efficiency in each device (anaerobic promoters particularly), Sugarfilm would benefit a lot.

Improvements listed above may be subtle, yet they all mean a lot to the whole system. Sugarfilm, with its versatility and feasibility, has great vista in application in synthesizing work flow. By every small step towards the perfect, we believe Sugarfilm would outstand in the end, as a truly beneficial and powerful method in industry.