Team:ZJU-China/data.html
From 2011.igem.org
Biofilm
Rainbofilm
Xfilm
Parts
Achievements
Tools
Data Page
Experiment Results
Biofilm Formation
Figure 1
The figure shows the modified DH5alpha's biofilm formed on rubber tube. The pink color was due to the RFP expressed by the bacteria. The picture was obtained with Leica DME microscope, under 40X objective.
Figure 2
3D CLSM picture of the biofilm formed by E. Coli DH5alpha. The picture was obtained with Zeiss LSM 710NLO confocal laser scanning microscope, using a 20X objective. The colors of the biofilm were not from the fluorescence proteins but artificially added in order to give better resolution.
Figure 3
CLSM image of the stratified biofilm obtained with the Cell Culture Plate Method. It was formed by our modified E.Coli expressing device BBa_K561003 (you can refer to our Rainbofilm module for more information). The picture was taken by Zeiss LSM510 Meta confocal laser scanning microscope, using a 63? oil-immersion objective.
Rainbofilm (Blue & Yellow)
Rainbofilm (Blue & Red)
Rainbofilm (Yellow & Red)
Rainbofilm (Rainbow)
Parts Characterization
BBa_I13602
The expression of CFP under aerobic and anaerobic conditions
BBa_K561000
The expression of YFP under aerobic conditions
The expression of YFP under anaerobic conditions
BBa_K561002
The expression of RFP under aerobic conditions
The expression of RFP under anaerobic conditions
The expression of RFP under micro-aerobic conditions (2.5%)
PoPS of RFP and the expression level of RFP
BBa_K561003
The expression of CFP repressed by tetR which is expressed by subpart of BBa_K561000 or BBa_K561002: