Template:Protocols

From 2011.igem.org

(Difference between revisions)
Line 26: Line 26:
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
<b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b>
<b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b>
-
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
+
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.<br><br>
-
</b></br></br></br></br>
+
<b><a href="/Team:British_Columbia/Protocols/Fungi">Yeast-Fungi Co-culture Experiments</a><br></b>
 +
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
 +
</br></br></br></br>
</html>
</html>

Revision as of 23:44, 14 October 2011

Team: British Columbia - 2011.igem.org


iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.

Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.