Template:Protocols

From 2011.igem.org

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<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b>
<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b>
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a><br></b>
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<b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b>
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
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Revision as of 06:31, 14 October 2011

Team: British Columbia - 2011.igem.org


iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.