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Template:Protocols
From 2011.igem.org
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<a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b> | <a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b> | ||
| - | Things to think about when designing your project and experiments, as well as general safety rules.<br> | + | Things to think about when designing your project and experiments, as well as general safety rules.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b> | ||
| - | A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br> | + | A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b> | ||
| - | How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br> | + | How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b> | ||
| - | How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br> | + | How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b> | ||
| - | A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br> | + | A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a><br></b> | ||
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector. | Preliminary experiments to probe the efficiency of transferring yeast via beetle vector. | ||
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Revision as of 02:53, 14 October 2011
iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.
Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.
Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.
Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.
Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
