"
Template:Protocols
From 2011.igem.org
| Line 20: | Line 20: | ||
Things to think about when designing your project and experiments, as well as general safety rules.<br> | Things to think about when designing your project and experiments, as well as general safety rules.<br> | ||
<a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br> | <a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br> | ||
| - | <br> | + | A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br> |
<a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br> | <a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br> | ||
| - | <br> | + | How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br> |
<a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br> | <a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br> | ||
| - | <br> | + | How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br> |
<a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br> | <a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br> | ||
| - | <br> | + | A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br> |
<a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a><br> | <a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a><br> | ||
| - | + | Preliminary experiments to probe the efficiency of transferring yeast via beetle vector. | |
</b> | </b> | ||
</html> | </html> | ||
Revision as of 02:49, 14 October 2011
iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.
Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.
Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.
Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.
Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
