Template:Protocols

From 2011.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 3: Line 3:
<html><style>
<html><style>
#logo {width:150px; float:left; background-color: white; margin-left: 8px;  margin-top:10px;}
#logo {width:150px; float:left; background-color: white; margin-left: 8px;  margin-top:10px;}
-
</style>
+
#other {width:780px; float:left; background-color: white; margin-left: 8px;  margin-top:10px;}
 +
#bod {width:950px; float:left; background-color: white; margin-left: 8px;  margin-top:10px;}</style>
 +
<div id="bod">
<div id="logo">
<div id="logo">
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/a/a8/Ubcwk1.jpg" >
+
<img align="left" style="margin-bottom:20px; padding:0;" src="https://static.igem.org/mediawiki/2011/9/96/Ubcwk4.jpg" >
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/3/34/Ubcwk8.jpg" >
+
<img align="left" style="margin-bottom:20px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/19/Ubcsmilie.jpg" >
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/66/Ubcwk2.jpg" >
+
<img align="left" style="margin-bottom:20px; padding:0;" src="https://static.igem.org/mediawiki/2011/b/bb/Ubcigemhearttips.jpg" >
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/9/96/Ubcwk4.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/a/a3/Ubcwk3.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/19/Ubcsmilie.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/9/98/Ubcigemwk10.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/8/82/Ubcwk9.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/f/f8/Ubcwk21.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/b/bf/Ubcwk5.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/0/0d/Ubcigemyeastransform.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/e/e4/Ubcwk6.jpg" >
+
-
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/c/c2/Ubcigemgcms.jpg" >
+
 +
</div>
 +
<div id="other">
 +
<a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b>
 +
Things to think about when designing your project and experiments, as well as general safety rules.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b>
 +
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b>
 +
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b>
 +
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b>
 +
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b>
 +
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.<br><br>
 +
<b><a href="/Team:British_Columbia/Protocols/Fungi">Yeast-Fungi Co-culture Experiments</a><br></b>
 +
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
 +
</br></br></br>
</div></html>
</div></html>
-
 
-
<html></br></br></br></br></br></br>
 
-
<a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br>
 
-
<a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br>
 
-
<a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br>
 
-
<a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br>
 
-
<a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br>
 
-
<a href="/Team:British_Columbia/Protocols/Yeast">Beetle Transfer Experiments</a>
 
-
</b>
 
-
</html>
 

Latest revision as of 17:46, 18 October 2011

Team: British Columbia - 2011.igem.org

iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.

Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.