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Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy
From 2011.igem.org
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<b>2.</b> Add 100µL of bacteria from a saturated liquid culture. | <b>2.</b> Add 100µL of bacteria from a saturated liquid culture. | ||
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<b>3.</b> Finally, add 3 or 4 sterile glass slides with a sterile tweezer. | <b>3.</b> Finally, add 3 or 4 sterile glass slides with a sterile tweezer. | ||
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<b>4.</b> Incubate at 30°C overnight. | <b>4.</b> Incubate at 30°C overnight. | ||
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Revision as of 11:23, 15 September 2011
Microscopy test
This protocol aims at
Procedure
1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
2. Add 100µL of bacteria from a saturated liquid culture.
3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
4. Incubate at 30°C overnight.
4. Observe the slides with a fluorescent microscope.
