Team:Lyon-INSA-ENS/Realisation/Collection

From 2011.igem.org






Plasmids collection
Collection reference Description Resistance(s)
pIG1 phaCAB -
pIG2 rcn-curli cloned in pUC57 plasmid Amp
pIG3 Pfort (18A: BBa_J23119) Amp
pIG4 part Bba_K342000 = Pcurli Cm
pIG5 enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) Amp+K
pIG6 pUC18 Amp
pIG7 pUC18 Amp
pIG8 Weak RBS (5L: Bba_J61101) Amp
pIG9 GFP with LVA tag (2L: BBa_K145015) Amp
pIG10 RBS-YFP (22M: BBa_I15017) Amp+K
pIG11 OmpR234 (BBa_K342003) Cm
pIG13 RBS-GFP-LVA (BBa_K206009) Cm
pIG14 Strong RBS (2M: BBa_B0034) Amp
pIG15 NiCoT Amp
pIG16 18A-OmpR234 (pIG3+pIG11) Amp
pIG17 Strong RBS-YFP (pIG14+pIG10) (2M24E) Amp
pIG18 RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) T
pIG19 RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) K
pIG20 Prcn-csg extracted in pUC57 () Amp
pIG21 Backbone A = pSB1A3 Amp
pIG22 Backbone K = pSB1K3 K
pIG23 Backbone T = pSB1T3 T
pIG24 Backbone C = pSB1C3 Cm
pIG25 Synthetic part cloned in pSB1C3 Cm
pIG26 part Prcn (= rcnA promoter) Cm
pIG27 part Prcn (= rcnA promoter) Cm
pIG28 part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) Cm
pIG29 part CsgBA Amp
pIG30 pIG25 -
pIG31 pKK-OmpR234 (p56 collection biofilm) Amp
pIG32 pPROBE OT'-Prcn-GFP (p157 collection biofilm) Spc
pIG33 PrcnA-OmpR234 Cm
pIG34 PrcnA-OmpR234 K
pIG35 = pIG25 Cm




Strains collection
Collection reference Description Phenotype
S5 Amp resistant
S6 NM522/pIG26 Cm resistant
S7 MC4100 streptomycin resistant
S8 MG1655 -
S9 NM522/pIG27 Cm resistant
S10 NM522/pIG26 Cm resistant
S11 NM522 -
S12 DH5α nalidixic acid resistant
S13 NMYO Kan resistant
S14 NM522 + pTNiCo Amp resistant, pTNiCo : NiCo transporter from Novosphingobium aromaticivorans inserted in pUC19
S15 PHL818 Tet resistant, sticky
S16 PHL1273 Tet resistant, Kan resistant
S17 NM522/pIG25 Cm resistant
S18 NM522/pIG2 Amp resistant
S19=S20 NM522/pIG16 Amp resistant
S21 NM522/pIG6 AmpR
S22 NM522/pIG33 -
S23 PHL1414/pIG2 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S24 PHL1414/pIG6 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S25 MC4100/piG6 AmpR
S26 MC4100/piG2 AmpR
S27 MC4100/pIG27 CmR
S28 MC4100/pIG16 AmpR
S29 PHL1414 CmR (genomic), constitutively fluorescent
S30 PHL1414/pIG3 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S31 PHL1414/pIG16 CmR (genomic), AmpR (plasmidic). Constitutively fluorescent
S32 MC4100/pIG34 KanR
S33 MC4100/pIG33 CmR





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