Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy

From 2011.igem.org






Microscopy test





This protocol aims at preparing glass slides for observation of biofilms with a fluorescent microscope.





Procedure



1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.


2. Add 100µL of bacteria from a saturated liquid culture.


3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.


4. Incubate at 30°C overnight.


5. Observe the slides with a fluorescent microscope.




























































































































ENS assystem Biomérieux INSA INSA