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From 2011.igem.org

September 25th - October 1st

Sunday, October 9

iGEM 2011 Regional Jamborees: Americas!

Monday, October 10

iGEM 2011 Regional Jamborees: Americas Award Ceremony

• Cornell received Gold Award
• Cornell advances to the iGEM 2011 World Jamborees!

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Outreach

• Attended meetings for both AlumniGEM and Community Bricks.

Tuesday, October 11

Wednesday, October 12

Lab work done by: Charlie Chung

• Picked colonies: five (40mL LB + 40µL ampicillin) cultures shaking at 37°C

- AviTagged GFP

- Dysfunctional RFP colony (negative control)

- AviTagged VioA from PCR deletion

- AviTagged VioB from PCR deletion

- AviTagged VioE from PCR deletion

Thursday, October 13

Lab work done by: Charlie Chung

• Freshly made 1000x ampicillin and 1M IPTG are in the DNA box of Olin 301 (-20°C fridge)

• 20mL from 40mL cultures of VioA, B, E were transferred to (1L LB + 1mL ampicillin) flask for a 1:50 dilution

- Wanted OD to be between 0.05 and 0.08 after a 2-3hr incubation period, but accidentally let it grow over to 0.1xx

- Regardless, induced with 1mL 1M IPTG

- Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down)

• two 10mL from 40mL culture of GFP were transferred to two (500mL LB + 500µL ampicillin) flasks for a 1:50 dilution

         o Wanted OD to be between 0.05 and 0.08 after a 2-3hr incubation period, but accidentally let it grow over to 0.1xx
         o Regardless, induced with 500µL 1M IPTG
         o Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down)

   * Took 500µL from remainder of the 40mL cultures and added 500µL of 30% glycerol to create freezer stocks of GFP and VioA, B, E. In -80°C freezer of Olin 303 -- Didi knows location.

   * Miniprep purification of 5mL from remainder of the 40mL cultures. Eluted products are in -20°C fridge. To be submitted for sequencing

Friday, October 14

Saturday, October 15