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Team:Cornell/Week 19
From 2011.igem.org
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Lab work done by: Charlie Chung | Lab work done by: Charlie Chung | ||
:*Freshly made 1000x ampicillin and 1M IPTG are in the DNA box of Olin 301 (-20°C fridge) | :*Freshly made 1000x ampicillin and 1M IPTG are in the DNA box of Olin 301 (-20°C fridge) | ||
| + | |||
:*20mL from 40mL cultures of VioA, B, E were transferred to (1L LB + 1mL ampicillin) flask for a 1:50 dilution | :*20mL from 40mL cultures of VioA, B, E were transferred to (1L LB + 1mL ampicillin) flask for a 1:50 dilution | ||
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::- Regardless, induced with 1mL 1M IPTG | ::- Regardless, induced with 1mL 1M IPTG | ||
::- Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down) | ::- Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down) | ||
| + | |||
:*two 10mL from 40mL culture of GFP were transferred to two (500mL LB + 500µL ampicillin) flasks for a 1:50 dilution | :*two 10mL from 40mL culture of GFP were transferred to two (500mL LB + 500µL ampicillin) flasks for a 1:50 dilution | ||
| - | + | ::- Wanted OD to be between 0.05 and 0.08 after a 2-3hr incubation period, but accidentally let it grow over to 0.1xx | |
| - | + | ::- Regardless, induced with 500µL 1M IPTG | |
| - | + | ::- Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down) | |
| + | |||
| - | + | :*Took 500µL from remainder of the 40mL cultures and added 500µL of 30% glycerol to create freezer stocks of GFP and VioA, B, E | |
| + | ::- In -80°C freezer of Olin 303 | ||
| - | + | :*Miniprep purification of 5mL from remainder of the 40mL cultures. Eluted products are in -20°C fridge. To be submitted for sequencing | |
==Friday, October 14== | ==Friday, October 14== | ||
==Saturday, October 15== | ==Saturday, October 15== | ||
Revision as of 19:13, 14 October 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 25th - October 1st
Sunday, October 9
iGEM 2011 Regional Jamborees: Americas!
Monday, October 10
- iGEM 2011 Regional Jamborees: Americas Award Ceremony
- Cornell received Gold Award
- Cornell advances to the iGEM 2011 World Jamborees!
- Outreach
- Attended meetings for both AlumniGEM and Community Bricks.
Tuesday, October 11
Wednesday, October 12
Lab work done by: Charlie Chung
- Picked colonies: five (40mL LB + 40µL ampicillin) cultures shaking at 37°C
- - AviTagged GFP
- - Dysfunctional RFP colony (negative control)
- - AviTagged VioA from PCR deletion
- - AviTagged VioB from PCR deletion
- - AviTagged VioE from PCR deletion
Thursday, October 13
Lab work done by: Charlie Chung
- Freshly made 1000x ampicillin and 1M IPTG are in the DNA box of Olin 301 (-20°C fridge)
- 20mL from 40mL cultures of VioA, B, E were transferred to (1L LB + 1mL ampicillin) flask for a 1:50 dilution
- - Wanted OD to be between 0.05 and 0.08 after a 2-3hr incubation period, but accidentally let it grow over to 0.1xx
- - Regardless, induced with 1mL 1M IPTG
- - Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down)
- two 10mL from 40mL culture of GFP were transferred to two (500mL LB + 500µL ampicillin) flasks for a 1:50 dilution
- - Wanted OD to be between 0.05 and 0.08 after a 2-3hr incubation period, but accidentally let it grow over to 0.1xx
- - Regardless, induced with 500µL 1M IPTG
- - Shaking at room temp in Olin 301 for 20+ hours until 6pm on Friday (when we'll spin the cells down)
- Took 500µL from remainder of the 40mL cultures and added 500µL of 30% glycerol to create freezer stocks of GFP and VioA, B, E
- - In -80°C freezer of Olin 303
- Miniprep purification of 5mL from remainder of the 40mL cultures. Eluted products are in -20°C fridge. To be submitted for sequencing
