Team:British Columbia/Protocols/Fungi

From 2011.igem.org

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==Yeast Fungi Co-culture Experiment==
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==Yeast Blue Stain Fungi Co-culture Experiment==
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1) Place mycelium plug of BSF in centre of OMEA or YPD plate. Streak out appropriate yeast in a ring around the fungus.
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2) Allow to grow for several days.

Latest revision as of 01:16, 29 October 2011

Team: British Columbia - 2011.igem.org

iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.

Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.


Yeast Blue Stain Fungi Co-culture Experiment

1) Place mycelium plug of BSF in centre of OMEA or YPD plate. Streak out appropriate yeast in a ring around the fungus.

2) Allow to grow for several days.