Team:British Columbia/Accomplishments

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We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.
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===Human Practice===
===Human Practice===

Revision as of 00:12, 28 October 2011

Team: British Columbia - 2011.igem.org

Project Accomplishments

Wet Laboratory Results

1. In vitro assay production of monoterpene in bacteria

Geranyl Pyrophosphate (GPP) Assay


Alpha-beta pinene and Beta-pinene synthases were The purified enzymes were assayed in vitro with GPP and sent for GC-MS, which confirmed alpha- and beta-pinene as products.


Ubcigem2011betapingcms.jpg

Modeling Results

Monoterpene Production vs Time in Pinene Synthase.jpg

We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.