Team:British Columbia/Accomplishments

From 2011.igem.org

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Revision as of 02:33, 28 October 2011

Team: British Columbia - 2011.igem.org

Contents

Wet Laboratory Achievements

1. In vitro assay production of monoterpene in bacteria

Geranyl Pyrophosphate (GPP) Assay

Alpha-pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS), we confirmed the synthesis of alpha and beta pinene monoterpenes from our enzyme assays.


Ubcigem2011betapingcms.jpg


2. Diterpene production in yeast

3. Mountain Pine Beetle & Yeast Co-culture

To prove that release methods by incubation of Mountain Pine Beetles

3. Blue Stain Fungi & Yeast Co-culture Co-culture

Modeling Achievements


Monoterpene Production

Monoterpene Production vs Time in Pinene Synthase.jpg


We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.