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Team:Washington/Protocols/Digestion
From 2011.igem.org
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Revision as of 01:32, 14 September 2011
General Digestion Protocol
10 uL DNA
5uL buffer ( #2 for most, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp)
.5 uL BSA
1uL enzyme 1
1uL enzyme 2
water to 50 uL (32.5 uL, add first)
