General Digestion Protocol
10 uL DNA
5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
0.5 uL BSA (if required. See above)
1uL enzyme 1
water to 50 uL (32.5 uL, add first)
If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.