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Team:Washington/Protocols/Digestion
From 2011.igem.org
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10 uL DNA | 10 uL DNA | ||
| - | 5uL buffer ( 2 for most, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp) | + | 5uL buffer ( #2 for most, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp) |
.5 uL BSA | .5 uL BSA | ||
Revision as of 01:31, 14 September 2011
General Digestion Protocol
10 uL DNA
5uL buffer ( #2 for most, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp)
.5 uL BSA
1uL enzyme 1
1uL enzyme 2
water to 50 uL(32.5 uL, add first)
