Team:UNIPV-Pavia/Freezer

From 2011.igem.org

(Difference between revisions)
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   </tr>
   </tr>
 +
</table>
 +
 +
 +
 +
<table class="data">
 +
    <tr>
 +
      <td class="row"><b>Biobrick name</b></td>
 +
      <td class="row"><b>Description</b></td>
 +
      <td class="row"><b>Ligation</b></td>
 +
      <td class="row"><b>Length</b></td>
 +
      <td class="row"><b>Strain</b></td>
 +
      <td class="row"><b>Vector</b></td>
 +
      <td class="row"><b>Quality Control</b></td>
 +
      <td class="row"><b>Creation Date</b></td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E1-2</td>
 +
      <td class="row">aiiA-TT</td>
 +
      <td class="row">C0060(E-S)+B0015(E-X)</td>
 +
      <td class="row">926</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1AK3</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E2-2</td>
 +
      <td class="row">RBS30-LuxI</td>
 +
      <td class="row">C0061(X-P)+B0030(S-P)</td>
 +
      <td class="row">639</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E3-1</td>
 +
      <td class="row">RBS31-LuxI</td>
 +
      <td class="row">C0061(X-P)+B0031(S-P)</td>
 +
      <td class="row">638</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E4-2</td>
 +
      <td class="row">RBS32-LuxI</td>
 +
      <td class="row">C0061(X-P)+B0032(S-P)</td>
 +
      <td class="row">637</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E5-2</td>
 +
      <td class="row">RBS30-mRFP-TT</td>
 +
      <td class="row">I13501(X-P)+B0030(S-P)</td>
 +
      <td class="row">864</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E6-1</td>
 +
      <td class="row">RBS31-mRFP-TT</td>
 +
      <td class="row">I13501(X-P)+B0031(S-P)</td>
 +
      <td class="row">863</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E7-2</td>
 +
      <td class="row">RBS32-mRFP-TT</td>
 +
      <td class="row">I13501(X-P)+B0032(S-P)</td>
 +
      <td class="row">862</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E8-3</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
 +
      <td class="row">K081022(E-P)+4C5(E-P)</td>
 +
      <td class="row">969</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Length OK</td>
 +
      <td class="row">07/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E9-2</td>
 +
      <td class="row">RBS30-aiiA-TT</td>
 +
      <td class="row">E1(X-P)+B0030(S-P)</td>
 +
      <td class="row">947</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E10-1</td>
 +
      <td class="row">RBS31-aiiA-TT</td>
 +
      <td class="row">E1(X-P)+B0031(S-P)</td>
 +
      <td class="row">946</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E11-1</td>
 +
      <td class="row">RBS32-aiiA-TT</td>
 +
      <td class="row">E1(X-P)+B0032(S-P)</td>
 +
      <td class="row">945</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">14/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E13-1</td>
 +
      <td class="row">pTet-RBS30-LuxI</td>
 +
      <td class="row">E2(X-P)+R0040(S-P)</td>
 +
      <td class="row">699</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E14</td>
 +
      <td class="row">pTet-RBS31-LuxI</td>
 +
      <td class="row">E3(X-P)+R0040(S-P)</td>
 +
      <td class="row">700</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row"></td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E15</td>
 +
      <td class="row">pTet-RBS32-LuxI</td>
 +
      <td class="row">E4(X-P)+R0040(S-P)</td>
 +
      <td class="row">699</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row"></td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E16-1</td>
 +
      <td class="row">pTet-RBS34-LuxI</td>
 +
      <td class="row">C0261(X-P)+R0040(S-P)</td>
 +
      <td class="row">698</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E17-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
 +
      <td class="row">E5(X-P)+E8(S-P)</td>
 +
      <td class="row">1839</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E18-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
 +
      <td class="row">E6(X-P)+E8(S-P)</td>
 +
      <td class="row">1840</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E19-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
 +
      <td class="row">E7(X-P)+E8(S-P)</td>
 +
      <td class="row">1839</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E20-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
 +
      <td class="row">I13507(X-P)+E8(S-P)</td>
 +
      <td class="row">1838</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E17-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
 +
      <td class="row">E5(X-P)+E8(S-P)</td>
 +
      <td class="row">1839</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E18-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
 +
      <td class="row">E6(X-P)+E8(S-P)</td>
 +
      <td class="row">1840</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E19-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
 +
      <td class="row">E7(X-P)+E8(S-P)</td>
 +
      <td class="row">1839</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E20-2</td>
 +
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
 +
      <td class="row">I13507(X-P)+E8(S-P)</td>
 +
      <td class="row">1838</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E21-1</td>
 +
      <td class="row">pTet-RBS30-mRFP-TT</td>
 +
      <td class="row">E5(X-P)+R0040(S-P)</td>
 +
      <td class="row">924</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">14/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E22-2</td>
 +
      <td class="row">pTet-RBS31-mRFP-TT</td>
 +
      <td class="row">E6(X-P)+R0040(S-P)</td>
 +
      <td class="row">925</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">13/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E23-1</td>
 +
      <td class="row">pTet-RBS32-mRFP-TT</td>
 +
      <td class="row">E7(X-P)+R0040(S-P)</td>
 +
      <td class="row">924</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">14/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E24-2</td>
 +
      <td class="row">pTet-RBS30-aiiA-TT</td>
 +
      <td class="row">E9(X-P)+R0040(S-P)</td>
 +
      <td class="row">1007</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E25-1</td>
 +
      <td class="row">pTet-RBS31-aiiA-TT</td>
 +
      <td class="row">E10(X-P)+R0040(S-P)</td>
 +
      <td class="row">1008</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E26-2</td>
 +
      <td class="row">pTet-RBS32-aiiA-TT</td>
 +
      <td class="row">E11(X-P)+R0040(S-P)</td>
 +
      <td class="row">1007</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E27-2</td>
 +
      <td class="row">pTet-RBS34-aiiA-TT</td>
 +
      <td class="row">E12(X-P)+R0040(S-P)</td>
 +
      <td class="row">1006</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB1A2</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E28-1</td>
 +
      <td class="row">pTet-RBS30-luxI in pSB4C5</td>
 +
      <td class="row">E13(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">699</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E29</td>
 +
      <td class="row">pTet-RBS31-luxI in pSB4C5</td>
 +
      <td class="row">E14(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">700</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">???</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E30</td>
 +
      <td class="row">pTet-RBS32-luxI in pSB4C5</td>
 +
      <td class="row">E15(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">699</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">???</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E31-1</td>
 +
      <td class="row">pTet-RBS34-luxI in pSB4C5</td>
 +
      <td class="row">E16(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">698</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E32-2</td>
 +
      <td class="row">pTet-RBS30-mRFP-TT in pSB4C5</td>
 +
      <td class="row">E21(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">924</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E33-2</td>
 +
      <td class="row">pTet-RBS31-mRFP-TT in pSB4C5</td>
 +
      <td class="row">E22(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">925</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E34-1</td>
 +
      <td class="row">pTet-RBS32-mRFP-TT in pSB4C5</td>
 +
      <td class="row">E23(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">924</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E35-2</td>
 +
      <td class="row">pTet-RBS34-mRFP-TT in pSB4C5</td>
 +
      <td class="row">I13521(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">923</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Seq ok (BMR Genomics)</td>
 +
      <td class="row">21/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E36</td>
 +
      <td class="row">pTet-J61002 in pSB4C5</td>
 +
      <td class="row">pTet-J61002(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">923</td>
 +
      <td class="row">E. coli TOP10</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row">Red colonies</td>
 +
      <td class="row">25/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E37-2</td>
 +
      <td class="row">pTet-RBS30-aiiA-TT in pSB4C5</td>
 +
      <td class="row">E24(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">1007</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E38-1</td>
 +
      <td class="row">pTet-RBS31-aiiA-TT in pSB4C5</td>
 +
      <td class="row">E25(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">1008</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E39-1</td>
 +
      <td class="row">pTet-RBS32-aiiA-TT in pSB4C5</td>
 +
      <td class="row">E26(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">1007</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E40-2</td>
 +
      <td class="row">pTet-RBS34-aiiA-TT in pSB4C5</td>
 +
      <td class="row">E27(E-P)+pSB4C5(E-P)</td>
 +
      <td class="row">1006</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E41N1</td>
 +
      <td class="row">pTet-RBS31-luxI in pSB4C5</td>
 +
      <td class="row">E36(S-P)+E3(X-P)</td>
 +
      <td class="row">700</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">04/08/11</td>
 +
  </tr>
 +
 +
  <tr>
 +
      <td class="row">E42-1</td>
 +
      <td class="row">pTet-RBS32-luxI in pSB4C5</td>
 +
      <td class="row">E36(S-P)+E4(X-P)</td>
 +
      <td class="row">699</td>
 +
      <td class="row">E. coli MGZ1</td>
 +
      <td class="row">pSB4C5</td>
 +
      <td class="row"></td>
 +
      <td class="row">29/07/11</td>
 +
  </tr>
</table>
</table>
</center>
</center>
 +
 +
 +

Revision as of 09:03, 19 August 2011

UNIPV TEAM 2011

FREEZER


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969 E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/11
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789 E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/11
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618 E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/11
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) 2009 13 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923 E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661 E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/11
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/11
Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 926 E. coli TOP10 pSB1AK3 Length OK 07/07/11
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 639 E. coli TOP10 pSB1A2 Length OK 07/07/11
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 638 E. coli TOP10 pSB1A2 Length OK 07/07/11
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 637 E. coli TOP10 pSB1A2 Length OK 07/07/11
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864 E. coli TOP10 pSB1A2 Length OK 07/07/11
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863 E. coli TOP10 pSB1A2 Length OK 07/07/11
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862 E. coli TOP10 pSB1A2 Length OK 07/07/11
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969 E. coli TOP10 pSB4C5 Length OK 07/07/11
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 947 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/11
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 946 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/11
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 945 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/11
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 699 E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/11
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 700 E. coli TOP10 pSB1A2 13/07/11
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 699 E. coli TOP10 pSB1A2 13/07/11
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 698 E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/11
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1839 E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/11
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840 E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/11
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839 E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/11
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838 E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/11
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1839 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/11
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/11
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/11
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/11
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 924 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/11
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/11
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/11
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1007 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/11
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1008 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/11
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1007 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/11
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1006 E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/11
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 699 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 700 E. coli MGZ1 pSB4C5 ???
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 699 E. coli MGZ1 pSB4C5 ???
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 698 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 924 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923 E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/11
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923 E. coli TOP10 pSB4C5 Red colonies 25/07/11
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1007 E. coli MGZ1 pSB4C5 29/07/11
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1008 E. coli MGZ1 pSB4C5 29/07/11
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1007 E. coli MGZ1 pSB4C5 29/07/11
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1006 E. coli MGZ1 pSB4C5 29/07/11
E41N1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 700 E. coli MGZ1 pSB4C5 04/08/11
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+E4(X-P) 699 E. coli MGZ1 pSB4C5 29/07/11

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