June, 6th
These BioBrick were resuspended in 15ul ddH20:
- BBa_E2050 (mOrange, 744bp) 2010 Kit Plate 2, well 13N in pSB2K3
- BBa_K165018 (ADH1 terminator, 253 bp) 2010 Kit Plate 3, well 2M, J63009 (Amp)
- BBa_K165037 (tef2 promoter, 403bp) 2010 Kit Plate 3, well 22O, pSB1AK3
- BBa_P1004 (Chloramphenicol resistence cassette, 769 bp) 2009 Kit plate 1, well 7B, pSB1A1
- BBa_J61001 (R6K origin, 406bp) 2010 Kit plate 1, well 240, pSB1A2
- BBa_K081008 (RBS-luxI, 664bp) 2010 Kit plate 2, well 10L, pSB1A2
- BBa_K125500 (GFP fusion brick, 718bp) 2010 Kit plate 3, well 2P, pSB1A2
1,5 ul of each culture was transformed in 100 ul of home-made competent E. coli DH5alpha.
Tranformants were all plated on LB agar plates added with Ampicillin, except for BBa_E2050 (Kanamycin).
500 ml LB+Amp was prepared and 21 LB agar plates + Amp were prepared (500 ml).
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