Team:UNIPV-Pavia/Calendar/July/settimana4

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UNIPV TEAM 2011

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JULY: WEEK 4

July, 18th

Digestions of previously purified plasmids were performed for ligations:

Plasmid Kind DNA (μl) H2O (μl) Enzyme 1 (μl) Enzyme 2 (μl) Buffer H (μl) Final Volume (μl)
E9-2 Insert 11.5 9 1 Xbal 1 Pstl 2.5 25
E10-1 Insert 10 10.5 1 Xbal 1 Pstl 2.5 25
E11-1 Insert 4 16.5 1 Xbal 1 Pstl 2.5 25
E12-1 Insert 10 10.5 1 Xbal 1 PstI 2.5 25
E13-1 ??Insert?? 6 14.5 1 EcoRl 1 Pstl 2.5 25
E16-1 ??Insert?? 4 16.5 1 EcoRl 1 Pstl 2.5 25
E21-1 ??Insert?? 7.5 13 1 EcoRl 1 Xbal 2.5 25
E22-2 ??Insert?? 10 10.5 1 EcoRl 1 Pstl 2.5 25
<partinfo>BBa_I13521</partinfo> Insert 12.5 8 1 EcoRl 1 Pstl 2.5 25
<partinfo>BBa_R0040</partinfo>-<partinfo>BBa_J61002</partinfo> Vector 20.5 0 1 EcoRl 1 Pstl 2.5 25

Reactions were incubated at 37°C for three hours; meanwhile two medium-size agarose gels were prepared according to protocols. Moreover BBa_I13521 plasmid purification and quantification were carried out:

In the afternoon gel electrophoresis was performed.

Plasmid DNA (ng/μl)
<partinfo>BBa_I13521</partinfo> 37.3

2 righe sopra inserire l' immagine

As shown in figure, all clones were positive (apart from E13 run where it is possible to recognize only a band at approximately 2800 bp, corresponding to the undigested plasmid), so we cut and purified the bands of interest. Cells harbouring E13 were again inoculated in 5 ml LB + Amp.

E13 was newly then digested with restriction endonucleases:

Plasmid Kind DNA (μl) H2O (μl) Enzyme 1 (μl) Enzyme 2 (μl) Buffer H (μl) Final Volume (μl)
<partinfo>BBa_I13521</partinfo> Insert 1 19.5 1 EcoRl 1 Pstl 2.5 25

After gel extraction, cut DNA was quantified:

Plasmid DNA (ng/μl)
E9 (X-P) 5.9
E10 (X-P) 4.2
E11 (X-P) 5.2
E12 (X-P) 5.2
E16 (E-P) 6.4
E21 (E-P) 5.8
E22 (E-P) 6.5
E23 (E-P) 4.6
<partinfo>BBa_I13521</partinfo> 6.1
<partinfo>BBa_R0040</partinfo>-<partinfo>BBa_J61002</partinfo> 2.1

Then ligations were performed in a final volume of 10 μl:

Ligation Name Vector Vector volume (μl) Insert Insert volume (μl) Buffer (μl) T4 Ligase (μl)
E1 <partinfo>BBa_B0015</partinfo> (E-X) 1.5 <partinfo>BBa_C0060</partinfo> (E-S) 6.5 1 1
E2 <partinfo>BBa_B0030</partinfo> (S-P) 1.5 <partinfo>BBa_C0061</partinfo> (X-P) 6.5 1 1
E3 <partinfo>BBa_B0031</partinfo> (S-P) 1.5 <partinfo>BBa_C0061</partinfo> (X-P) 6.5 1 1
E4 <partinfo>BBa_B0032</partinfo> (S-P) 1.5 <partinfo>BBa_C0061</partinfo> (X-P) 6.5 1 1
E8 <partinfo>pSB4C5</partinfo> (E-P) 1.5 <partinfo>BBa_K081022</partinfo> (E-P) 6.5 1 1

Ligations were incubated ON at 16°C.

July, 19th