Team:UNIPV-Pavia

From 2011.igem.org

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                Abstract
 
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<div style="text-align:center"><div class="thumbinner" style="width: 800px;"><img src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage" width="89%"></a></div></div>
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<h2 class="art-postheader">The project</h2>
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<p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around  a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it.
<p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around  a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it.
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<img src="https://static.igem.org/mediawiki/2011/8/81/UNIPVteam1.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/f/fd/UNIPVteam2.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/1/13/UNIPV_team3.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/d/d3/UNIPV_Team4.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/2/2d/UNIPV_Hometeam1.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/0/0a/UNIPV_Hometeam2.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/e/e1/UNIPV_Hometeam3.jpg" title="UNIPV iGEM team" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/f/f2/UNIPV_Hometeam4.jpg" title="Work in progress" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/9/9b/UNIPV_Home1.jpg" title="Pavia and the river Ticino" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/a/a2/UNIPV_Home2.jpg" title="University of Pavia" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/f/f7/UNIPV_Home3.jpg" title="Faculty of Engineering" alt="" />
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<img src="https://static.igem.org/mediawiki/2011/f/f0/UNIPV_Home4.jpg" title="Scientific campus" alt="" />
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Latest revision as of 13:14, 19 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


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