Team:ETH Zurich/Achievements

From 2011.igem.org

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* We [[Team:ETH_Zurich/Process/Validation|systematically came up with the current channel design]] through a [[Team:ETH_Zurich/Process/Microfluidics|process of developing and refining chain of ideas for different designs]].
* We [[Team:ETH_Zurich/Process/Validation|systematically came up with the current channel design]] through a [[Team:ETH_Zurich/Process/Microfluidics|process of developing and refining chain of ideas for different designs]].
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* Modeling help to improved the channel design
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* Modeling help to improve the channel design
* We participated in the [[Team:ETH_Zurich/Process/Validation#Final Channel Construction|PDMS channel construction]].
* We participated in the [[Team:ETH_Zurich/Process/Validation#Final Channel Construction|PDMS channel construction]].
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Revision as of 03:13, 29 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi - Achievements

It has been a long four and a half months since we started working on our SmoColi project. During that time, we worked hard and learned a lot of new stuff. Below we summarize shortly what we did and what we have achieved so far. If you would like to read more about a particular achievement, click on the respective link.


Modeling


Biology

  • After severe cloning difficulties (due to unexpected lethality of active CI and LacI), the final system was completely established.
  • We improved the pSB6A1 Plasmid to standard 5: [http://partsregistry.org/Part:BBa_K625005 pSB6A5]
  • We created the [http://partsregistry.org/Part:BBa_K625000 BioBrick LacIM1] which is a codon modified LacI for multiplasmid operations
  • We created two improved versions of the PU BioBrick, characterized the PU promoter and fitted a dose response curve to the data
  • We characterized the PBAD dose response for homogenous expression of the PBAD in BW27783
  • We successfully tested the channel in terms of cell viability and GFP synthesis
  • We could establish a gradient of a small molecule (IPTG and arabinose) in a tube and also in the microfluidic channel


Microfluidics


Human Practices

  • We presented Synthetic Biology and iGEM to our fellow students in the CBB get together afternoon.
  • We presented iGEM and our project to the general public on the BSSE Open House Day through a poster and a slide show.
  • We also made a survey with critical questions related to Synthetic Biology and iGEM, asked people to fill it out and analyzed the results.
  • ETH GLOBE and NZZ (Neue Zeitung Zürich) will write about our participation at iGEM World Championship Jamboree
  • We helped the Edinburgh 2011 iGEM team with debugging their MATLAB cellulase model.


Collaboration


Personal achievements

  • xx managed to move the Dropbox folder to another location, thus making it look empty for the others.
  • xx accidentally modified an other teams wiki for 1 min
  • xx and xy slept the whole boat trip in Amsterdam
  • xy found a mistake in the PowerPoint in the morning before the presentation ("And the channel turns read")
  • xy managed to sleep at two chairs in the lab
  • We had fun!


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