Team:ETH Zurich/Achievements

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It has been long four and a half months since we started working on our SmoColi project. During that time, we worked hard and learned a lot of new stuff. Bellow we summarize shortly what we did and which we achievements we have so far. If you want to read more about them, click on the respective links.
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It has been long four and a half months since we started working on our SmoColi project. During that time, we worked hard and learned a lot of new stuff. Bellow we summarize shortly what we did and what we managed to achieve so far. If you want to read more about them, click on the respective links.
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Revision as of 03:05, 29 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi - Achievements

It has been long four and a half months since we started working on our SmoColi project. During that time, we worked hard and learned a lot of new stuff. Bellow we summarize shortly what we did and what we managed to achieve so far. If you want to read more about them, click on the respective links.


Modeling


Biology

  • After severe cloning difficulties (due to unexpected lethality of active CI and LacI), the final system was completely established.
  • We improved the pSB6A1 Plasmid to standard 5: [http://partsregistry.org/Part:BBa_K625005 pSB6A5]
  • We created the [http://partsregistry.org/Part:BBa_K625000 BioBrick LacIM1] which is a codon modified LacI for multiplasmid operations
  • We created two improved version of the PU BioBrick, characterized the PU promoter and fitted a dose response curve to the data
  • We characterized the PBAD dose response for homogenous expression of the PBAD in BW27783
  • We successfully tested the channel in terms of cell viability and GFP synthesis
  • We could established a gradient of a small molecule (IPTG and arabinose) in a tube and also in the microfluidic channel


Microfluidics


Human Practices

  • We presented Synthetic Biology and iGEM to our fellow students in the CBB get together afternoon.
  • We presented iGEM and our project to the general public on the BSSE Open House Day through a poster and a slide show.
  • We also made a survey with critical questions related to Synthetic Biology and iGEM, asked people to fill it out and analyzed the results.
  • ETH GLOBE and NZZ (Neue Zeitung Zürich) will write about our participation at iGEM World Championship Jamboree
  • We helped the Edinburgh 2011 iGEM team with debugging their MATLAB cellulase model.


Collaboration


Personal achievements

  • xx managed to move the Dropbox folder to another location, thus making it look empty for the others.
  • xx accidentally modified an other teams wiki for 1 min
  • xx and xy slept the whole boat trip in Amsterdam
  • xy found a mistake in the PowerPoint in the morning before the presentation ("And the channel turns read")
  • xy managed to sleep at two chairs in the lab
  • We had fun!


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