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Team:EPF-Lausanne/Protocols
From 2011.igem.org
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== Molecular Biology == | == Molecular Biology == | ||
| - | * [[Team:EPF-Lausanne/Protocols/ | + | === Products and stock preparation === |
| + | * [[Team:EPF-Lausanne/Protocols/Primers preparation|Primer preparation]]: initial dilution of ordered primers. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]: prepare cells for plasmid uptake. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]: introduce foreign plasmid into competent cells. | ||
| + | |||
| + | === Assembly === | ||
| + | |||
| + | * [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | ||
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | * [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | ||
| + | |||
| + | === Mutations === | ||
| + | |||
| + | * [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce site-specific mutations on tetR. | ||
| + | |||
| + | |||
| + | === DNA recovery === | ||
| + | |||
| + | * [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]: recover DNA separated by electrophoresis | ||
| + | |||
| + | |||
| + | |||
* [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce site-specific mutations on tetR. | * [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce site-specific mutations on tetR. | ||
* [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | * [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | ||
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* [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | ||
* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | ||
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== Microfluidics == | == Microfluidics == | ||
Revision as of 14:21, 13 July 2011
Protocols
Contents |
Molecular Biology
Products and stock preparation
- Primer preparation: initial dilution of ordered primers.
- Competent Cells: prepare cells for plasmid uptake.
- Competent Cells. Protocol II (Inoue method).
- Transformation: introduce foreign plasmid into competent cells.
Assembly
Mutations
- tetR mutation PCR: induce site-specific mutations on tetR.
DNA recovery
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Gel purification: recover DNA separated by electrophoresis
- tetR mutation PCR: induce site-specific mutations on tetR.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
- Competent Cells.
- Competent Cells. Protocol II (Inoue method).
- Transformation.
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
