"
Team:Bielefeld-Germany/Theory
From 2011.igem.org
| Line 7: | Line 7: | ||
===Bisphenol A degradation=== | ===Bisphenol A degradation=== | ||
| - | There exist a lot of bacteria in nature that can degrade xenobiotic substances such as phenolic compounds or endocrine disruptors. In a lot of soil samples that were taken from contaminated soil to find organisms that do so, ''sphingomonads'' were extraordinarily often isolated ([http://www.springerlink.com/content/t717l15u85507706/ Stolz, 2009]). In 2005, [http://www.springerlink.com/content/q7864l02734wg32m/ Sasaki ''et al.''] isolated a soil bacterium from the ''Sphingomonas'' genus which is able to degrade the endocrine disruptor bisphenol A (BPA) with a unique rate and efficiency compared to other BPA degrading organisms. This strain, called ''Sphingomonas bisphenolicum'' AO1, is able to completely decompose 120 mg BPA L<sup>-1</sup> in about 6 hours while other strains need days of cultivation (e.g. ''Sphingomonas'' strain BP-7 isolated by [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article Sakai ''et al.'' (2007)]). | + | There exist a lot of bacteria in nature that can degrade xenobiotic substances such as phenolic compounds or endocrine disruptors. In a lot of soil samples that were taken from contaminated soil to find organisms that do so, ''sphingomonads'' were extraordinarily often isolated ([http://www.springerlink.com/content/t717l15u85507706/ Stolz, 2009]). In 2005, [http://www.springerlink.com/content/q7864l02734wg32m/ Sasaki ''et al.''] isolated a soil bacterium from the ''Sphingomonas'' genus which is able to degrade the endocrine disruptor bisphenol A (BPA) with a unique rate and efficiency compared to other BPA degrading organisms. This strain, called ''Sphingomonas bisphenolicum'' AO1, is able to completely decompose 120 mg BPA L<sup>-1</sup> in about 6 hours while other strains need days of cultivation (e.g. ''Sphingomonas'' strain BP-7 isolated by [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article Sakai ''et al.'' (2007)]). The full bisphenol degradation pathway which is found in nature is shown in the following figure: |
| + | |||
| + | <html> | ||
| + | <img src="http://www.genome.jp/kegg/pathway/map/map00363.png" width="80%" height="80%" /> | ||
| + | </html> | ||
| + | |||
| + | Bisphenol A is mainly hydroxylated into the products 1,2-Bis(4-hydroxyphenyl)-2-propanol and 2,2-Bis(4-hydroxyphenyl)-1-propanol by some kind of oxidoreductase acting with NADH or NADPH. | ||
===Further applications of bisphenol A degrading BioBricks and enzymes=== | ===Further applications of bisphenol A degrading BioBricks and enzymes=== | ||
Revision as of 17:49, 7 September 2011
Contents |
Bisphenol A
Bisphenol A and its effects on mammals
bla bla bla
Bisphenol A degradation
There exist a lot of bacteria in nature that can degrade xenobiotic substances such as phenolic compounds or endocrine disruptors. In a lot of soil samples that were taken from contaminated soil to find organisms that do so, sphingomonads were extraordinarily often isolated ([http://www.springerlink.com/content/t717l15u85507706/ Stolz, 2009]). In 2005, [http://www.springerlink.com/content/q7864l02734wg32m/ Sasaki et al.] isolated a soil bacterium from the Sphingomonas genus which is able to degrade the endocrine disruptor bisphenol A (BPA) with a unique rate and efficiency compared to other BPA degrading organisms. This strain, called Sphingomonas bisphenolicum AO1, is able to completely decompose 120 mg BPA L-1 in about 6 hours while other strains need days of cultivation (e.g. Sphingomonas strain BP-7 isolated by [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article Sakai et al. (2007)]). The full bisphenol degradation pathway which is found in nature is shown in the following figure:
Bisphenol A is mainly hydroxylated into the products 1,2-Bis(4-hydroxyphenyl)-2-propanol and 2,2-Bis(4-hydroxyphenyl)-1-propanol by some kind of oxidoreductase acting with NADH or NADPH.
Further applications of bisphenol A degrading BioBricks and enzymes
S-layer
NAD+-detection
References
Sakai K, Yamanaka H, Moriyoshi K, Ohmoto T, Ohe T (2007) Biodegradation of Bisphenol A and Related Compounds by Sphingomonas sp. Strain BP-7 Isolated from Seawater, Bioscience, Biotechnology, and Biochemistry [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article 71(1):51-57].
Sasaki M, Maki J, Oshiman K, Matsumura Y, Tsuchido T (2005a) Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1, [http://www.springerlink.com/content/q7864l02734wg32m/ Biodegradation 16(5):449-459].
Stolz A (2009) Molecular characteristics of xenobiotic-degrading sphingomonads, Appl Microbiol Biotechnol [http://www.springerlink.com/content/t717l15u85507706/ 81:793-811].
