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From 2011.igem.org

Bisphenol A subproject: We enabled E. coli to degrade BPA in vivo and improved the specific BPA degradation rate by creating an Fd_bisd:CYP_bisd fusion protein, changing the cytochrome P450 electron transport system from a putidalike bacterial class I type to a class V type.

S-layer subproject: 4 different S-layer BioBricks with different lattice structures were created and sent to the partsregistry. The behaviour of these genes when expressed in E. coli were characterized and purification strategies for the expressed proteins were developed. Two purified fluorescent S-layer fusion proteins from different organisms were immobilized on beads, leading to a highly significant fluorescence enhancement of these beads (p < 10^-14).

NAD⁺ detection: The NAD⁺-dependent DNA ligase from E. coli (LigA) can be utilized for a highly sensitive molecular beacon based bioassay detecting NAD⁺ in nano molarity scale. The deadenylated form of LigA is able to ligate a split target hybridized to a molecular beacon resulting in an increase of fluorescence intensity which is still measurable in presence of 5 nM NAD⁺. Relating to this, the initial velocity of DNA ligation shows a linear dependence on the employed NAD⁺ concentration as long as this remains the limiting factor.