-Targeting-

-Digestion

Digested P0440 and K317037 with Pst1 and Xba1, then incubated for 7 hours at 37 deg C.

-Make gel to electrophorese

Made 100 ml

-Electrophoresis

Checked for P0440 and K317037

Results

Cut part of K317037

-Preculture

Precultured for P0440, K317037 and J23100, then incubated at 37 deg C.

-DNA refining

DNA refined J23100