Team:Tokyo Metropolitan/Notebook/A27

From 2011.igem.org

-Targeting-

-Digestion

Digested P0440 and K317037 with Pst1 and Xba1, then incubated for 7 hours at 37 deg C.


-Make gel to electrophorese

Made 100 ml


-Electrophoresis

Checked for P0440 and K317037


Results

Cut part of K317037


-Preculture

Precultured for P0440, K317037 and J23100, then incubated at 37 deg C.


-DNA refining

DNA refined J23100