Team:Osaka/week3

August 16 (Tue)

1. Gel electrophoresis of 01,02,03,04,05,06
2. Transfer to LB culture medium:01,02,03,04,05,06,1-23L
3. Ligation
   • 8/5 3. the same way
4. Transformation of Ligation products

August 17 (Wed)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Restriction digests of 03,05
5. Gel electrophoresis of digests
6. Ligation
   • 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
7. Transfer to LB culture midium:01,02,04,05,06

August 18 (Thu)

1. Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
2. Transformation of 07 and Resistry parts (See Table 3)

Table3

ID	Part Name	Resistance	Description
1-6I	<bbpart>BBa_R0040</bbpart>	A	TetR repressible promoter

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests

August 19 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Gel extraction of 1-1G,1-3A
5. Transfer to LB culture medium:1-6I,07

August 20 (Sat)

1. Gel electrophoresis of 1-5A
2. Gel extraction of 1-5A
3. Miniprep of yesterday's culture
4. Restriction digests of minipreppped parts
5. Gel electrophoresis of digests

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