Team:ETH Zurich
From 2011.igem.org
![](/wiki/images/3/39/ETHZ_FRONT_Slide_0.png)
![](/wiki/images/2/2e/ETHZ_FRONT_Slide_1.png)
![](/wiki/images/d/d6/ETHZ_FRONT_Slide_2.png)
![](/wiki/images/4/46/ETHZ_FRONT_Slide_3.png)
![](/wiki/images/d/d9/ETHZ_FRONT_Slide_4.png)
![](/wiki/images/1/1d/ETHZ_FRONT_Slide_5.png)
![](/wiki/images/e/ed/ETHZ_FRONT_Slide_7.png)
SmoColi - Abstract
We create a bacterio-quantifier of smoke. SmoColi cells are engineered to sense toxic substances found in cigarette smoke (for example acetaldehyde or xylene). They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red. Due to its modularity, our SmoColi system can be used in fact as a quantifier for a range of substances, as long as the sensor is adapted. To show that our system can be activated by different signals, as a proof of principle, we modified the circuit so that it can be induced by arabinose.