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AUGUST: WEEK 1
August, 1stFor each plate from the previous day, two colonies were picked. Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes.
Screening (E-S) bande 1-2-3. August, 2nd
MODIFICARE: una piccata per ciascuno degli J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate) Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:
MODIFICARE?: E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E42AGAIN-2, J101-31, J101-E5, J101-E7 were transformed in MGZ1 competent cells. Digestions of previously purified plasmids were performed for ligations. For each part (E28AGAIN-1, E28AGAIN-2, E41AGAIN-1 and E42AGAIN-2), the following aliquots for the various components were used:
MODIFICARE?: TOP10 competent cells, carrying T9002 construct, were transformed with ENTERO4C5.
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Team:UNIPV-Pavia/Calendar/August/week1
From 2011.igem.org
