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JULY: WEEK 5
July, 25th
Red colonies were observed from E36 plates, suggesting that the transformation was successful. A colony was picked form the plate. Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols. In the afternoon gel electrophoresis was performed. Immagine della corsa su gel After gel extraction, digested DNA was quantified:
Previously purified plasmids carrying either E17, or E18, or E19 or E20 part were inoculated in order to amplify them for the subsequent transformation in MGZ1 cells (E' GIUSTO?): July, 26th
Plasmids containing either E17, or E18, or E19, or E20 or E36 part were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:
Digestion of E36 part carrying plasmid was performed for ligation:
Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours while a small-size agarose gel was prepared according to protocols. In the afternoon gel electrophoresis was performed. Immagine della corsa su gel After gel extraction, digested DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
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Team:UNIPV-Pavia/Calendar/July/settimana5
From 2011.igem.org
