Team:UNIPV-Pavia/Calendar/July/settimana5

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UNIPV TEAM 2011

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July
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JULY: WEEK 5

July, 25th

Red colonies were observed from E36 plates, suggesting that the transformation was successful. A colony was picked form the plate.

Digestions of previously purified plasmids were performed for ligations:

Plasmid Kind DNA (μl) H2O (μl) Enzyme 1 (μl) Enzyme 2 (μl) Buffer H (μl) Final Volume (μl)
E24-2 Insert 13 7.5 1 Xbal 1 Pstl 2.5 25
E25-1 Insert 13.5 7 1 Xbal 1 Pstl 2.5 25
E26-2 Insert 14.5 6 1 Xbal 1 Pstl 2.5 25
E27-1 Insert 12.5 8 1 Xbal 1 PstI 2.5 25

Reactions were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols.

In the afternoon gel electrophoresis was performed.

Immagine della corsa su gel

After gel extraction, digested DNA was quantified:

Part DNA (ng/μl)
E24 (E-P) -
E25 (E-P) -
E26 (E-P) -
E27 (E-P) -