July, 25th
Digestions of previously purified plasmids were performed for ligations:
| Plasmid |
Kind |
DNA (μl) |
H2O (μl) |
Enzyme 1 (μl) |
Enzyme 2 (μl) |
Buffer H (μl) |
Final Volume (μl) |
| E9-2 |
Insert |
11.5 |
9 |
1 Xbal |
1 Pstl |
2.5 |
25 |
| E10-1 |
Insert |
10 |
10.5 |
1 Xbal |
1 Pstl |
2.5 |
25 |
| E11-1 |
Insert |
4 |
16.5 |
1 Xbal |
1 Pstl |
2.5 |
25 |
| E12-1 |
Insert |
10 |
10.5 |
1 Xbal |
1 PstI |
2.5 |
25 |
| E13-1 |
Insert |
6 |
14.5 |
1 EcoRl |
1 Pstl |
2.5 |
25 |
| E16-1 |
Insert |
4 |
16.5 |
1 EcoRl |
1 Pstl |
2.5 |
25 |
| E21-1 |
Insert |
7.5 |
13 |
1 EcoRl |
1 Xbal |
2.5 |
25 |
| E22-2 |
Insert |
10 |
10.5 |
1 EcoRl |
1 Pstl |
2.5 |
25 |
| BBa_I13521 |
Insert |
12.5 |
8 |
1 EcoRl |
1 Pstl |
2.5 |
25 |
| BBa_R0040-BBa_J61002 |
Vector |
20.5 |
0 |
1 EcoRl |
1 Pstl |
2.5 |
25 |
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