De lundi le 20 juin au vendredi le 24 juin 2011

Monday

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One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid

Tuesday

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4 more new clones have grown : start of 5mL liquid cultures on these clones.

Wednesday

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Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)

Thursday

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PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)

Friday

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