Du Lundi 25 Juillet au Vendredi 29 Juillet 2011

Lundi

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Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.

Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).

Mardi

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Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.

Mercredi

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Les PCR n'ont pas marché.

Jeudi

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Vendredi

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La PCR a finalement marché avec la Phusion.