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JUNE: WEEK 4
June, 23rdCultures incubated ON grew all. Plasmids were extracted with MiniPrep.
 After MiniPrep, purified DNA was quantified with NanoDrop.
Digestions were incubated at 37°C for 3 hours, gel run and gel-extracted. Ligations were performed ON at 16°C:
NanoDrop quantifications were not reliable, so every ligation was performed with 3ul of vector, 2ul of insert, 3ul of ddH20, 1ul of T4 ligase and 1ul of T4 ligase buffer. These four promoters from Anderson Promoters Collection were chose for their strength, measured in Arbitrary Units:
Soon quantitative tests will be performed to quantify each promoter's strength in terms of RPU. Today we received 2 stabs from iGEM HQ for BBa_K208001 (one for each registry location of this part), the Silver-fusion compatible BioBrick part that codes for phasin. This part is in a pSB3K3 plasmid. Bacteria were streaked on LB+Kan (50 ug/ml) agar plates. Cultures were named PhaP-1 and PhaP-2. Plates were incubated ON at 37°C.
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Team:UNIPV-Pavia/Calendar/June/settimana4
From 2011.igem.org
