Team:ETH Zurich/Process/Validation

From 2011.igem.org

Revision as of 18:54, 24 October 2011 by Smurmann (Talk | contribs)

Menu image preload Menu image preload Menu image preload Menu image preload Menu image preload Menu image preload


Final Setup and Validation
Experimental Setup Results Analysis
This page presents description of our final channel design as well as description of its construction, which we did by ourselves. Our final channel is build out of polydimethylsiloxane (PDMS) and constructed with a technique called photolithography. We are also presenting the experiments we did to validate our setup and to show that it can work in practice

Final channel construction

To construct our final channel, we used the technique photolithography. Photolithography is the photopatterning of channels from a mask (drawing of channels in 2D) and is based on the utilization of particular substances (photoresists) that become soluble to particular solvents after being exposed to UV light [1].



You can see below some photos of the channels and the channel building process:


Gallery: Producing the channel
ETH MasK mikro.png
Mikrofluidic negative.JPG
Negative and PDMS.JPG
Making holes.JPG
Cleaning.JPG
Plasmaofen.JPG

Within the devise we created small channels of different lengths ( and mm). The width of all of them is mm and their height is mm.


References: [1] http://www.elveflow.com/microfluidic/16-start-with-microfluidics

Setup Validation

bla

We checked whether our designed works by putting an engineered cells that produce GFP upon arabinose induction in agarose and filling the channel with it. In the reservoir we put arabinose in ____________ ?? After _____?? we optained a nice arabinose-inducible GFP gradient.

Figure 1: Proof of principle, arabinose-inducible GFP gradient created in the microfluidic PDMS devise
Retrieved from "http://2011.igem.org/Team:ETH_Zurich/Process/Validation"