Team:Tokyo Metropolitan/Notebook/S30

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-Visualization-

-oriTr-GFP＋oriTr Electophoresis to check for colony PCR

-oriTr-GFP＋oriTr Plasmid extraction to DNA refining

-oriTr-GFP＋oriTr Digestion

-oriTr-GFP＋oriTr Electrophoresis


Results

Ligation might be failed. Results from sequence, some samples lost oriTr.


-Colony PCR

Checked for results promoter - suppressed expression system (Cm)

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