Team:Tokyo Metropolitan/Notebook/S05

From 2011.igem.org

Revision as of 04:20, 5 October 2011 by Rosanium (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.

Amplified and purification I20260 with PCR.

Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S05"