"
Team:Tokyo Metropolitan/Notebook/S05
From 2011.igem.org
 |
 |
 |
 |
 |
 |
 |
 |
-Speeding up-
Digested HNS(wt) with XbaI+SpeI and I20260 with Blnâ… .
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
-Visualization-
-Ligation
J23100-I714051
-Transformation
J23100-I714051
-Plasmid extraction
Promoter and suppressed expression system
-DNA refining
Promoter and suppressed expression system
