Team:Osaka/week8

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Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Plating (cell survival)
Table 10
NameOD600UV(mJ/cm^2)
PprI-10.01090
PprI-20.01090.5
PprI-30.01091.0
PprI-40.01092.0
PprI-50.01095.0
PprA-10.04180
PprA-20.04180.5
PprA-30.04181.0
PprA-40.04182.0
PprA-50.04185.0
PprM-10.04540
PprM-20.04540.5
PprM-30.04541.0
PprM-40.04542.0
PprM-50.04545.0
RecA-10.01140
RecA-20.01140.5
RecA-30.01141.0
RecA-40.01142.0
RecA-50.01145.0
empty vector-10.05150
empty vector-20.05150.5
empty vector-30.05151.0
empty vector-40.05152.0
empty vector-50.05155.0
wild type-10.05460
wild type-20.05460.5
wild type-30.05461.0
wild type-40.05462.0
wild type-50.05465.0


September 20(Tue)

  1. To liquid culture:113,114
  2. colony check (cell survival)
Table 11
NameUV(mJ/cm^2)conoly counts
PprI-10
PprI-20.5
PprI-31.0
PprI-42.0
PprI-55.0
PprA-10
PprA-20.5
PprA-31.0
PprA-42.0
PprA-55.0
PprM-10
PprM-20.5
PprM-31.0
PprM-42.0
PprM-55.0
RecA-10
RecA-20.5
RecA-31.0
RecA-42.0
RecA-55.0
Empty vector-10
Empty vector-20.5
Empty vector-31.0
Empty vector-42.0
Empty vector-55.0
wild type-10
wild type-20.5
wild type-31.0
wild type-42.0
wild type-55.0
  1. Plating (cell survival)


September 21(Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 115(A):113(upstream)+114(downstream)+1-1G(vector)
  5. Transformation of ligation products
  6. colony check (cell survival)
  7. Plating (cell survival)


September 22(Thu)

  1. Colony check
  2. Plating
  3. To liquid culture

September 23(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Colony check

September 24(Sat)

  1. Preparation of LB plates
  2. Plating


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