Team:Arizona State/Lab/Protocols/Alkaline Phosphatase

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Protocols: Alkaline phosphatase


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Antarctic Alkaline Phosphatase

  • Immediately after restriction, add 5 uL phosphatase buffer to 50 uL restriction mix.
  • Add 1 uL Antarctic Phosphatase to mix.
  • Incubate for 15 minutes at 37C.
  • Heat kill at 65C for 5 minutes.
  • Proceed to ligation or store at -20C.

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