Team:Washington/Alkanes/Parts

From 2011.igem.org

The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].

Composite

[http://partsregistry.org/Part:BBa_K590025 BBa_K590025 "The Petrobrick"]

A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each behind a standard Elowitz RBS. All of this in psb1C3 with a high constitutive promoter.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590026 BBa_K590026 ADC-PSB1C3-High constitutive]

The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590027 BBa_K590027 AAR-PSB1C3-High consititutive]

The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

Coding

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase]

This part encodes an enzyme (Aldehyde Decarbonylase (ADC)) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 Acyl-ACP Reductase]

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.