The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the Parts Registry.
A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.
The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for E. coli.
This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.