Feasibility Analysis

In order for the GFP band to appear at the beginning of our channel, system is supplied with around 44.05 mg/L acetaldehyde. In order for the band to be able to form at the very end of the channel, the input acetaldehyde concentration is 2420 mg/L.

Process Design

• E. coli can generate a gradient by degrading a substance replenished by diffusion in an agarose-filled channel.
• It can do so within a reasonable experimental timeframe (within several hours).
• It can do so within reasonable channel lengths (several centimeters)
• Channel diameter is irrelevant for our system