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Results
Contents |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C. For the cloning of the parts, E. coli TOP10 was used.
Parts assembly
Characterization of basic modules
Characterization of promoters pTet and pLux
Characterization of enzymes AiiA and LuxI
Characterization of the efficiency of RBSs from the community collection
Identification of bacterial growth parameters
The bacterial growth curve has been modelled as a logistic curve and is represented by the following equation:
dN/dt=N*μ*(Nmax-N)/Nmax
N(0)=n0
where μ represents the growth rate of the cells (E. coli MGZ1 in M9 supplemented medium) and Nmax represents the maximum number of cells in the well. For a detailed description of the parameters, see modelling section. For details on parameters identification, see identification section.
| Culture | O.D.600 TECAN | O.D.600 Spectrophotometer | C.F.U. | Dilution Factor (10^) | N |
| C1 | 0,367950002 | 0,758004416 | 990 | 5 | 990000000 |
| C1 | 0,044649998 | 0,091982322 | 141 | 6 | 1410000000 |
| C1 | 0,004799999 | 0,009888356 | 136 | 5 | 136000000 |
| C1 | 0,000549998 | 0,001133037 | 20 | 6 | 200000000 |
| C2 | 0,54840003 | 1,129744917 | 165 | 4 | 16500000 |
| C2 | 0,058200002 | 0,119896339 | 23 | 5 | 23000000 |
| C2 | 0,008700002 | 0,017922652 | 251 | 3 | 2510000 |
| C2 | 0,000100002 | 0,000206011 | 24 | 4 | 2400000 |
