Team:Imperial College London/Reporters

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Reporters

Fluorescent reporters are an important tool in molecular biology, as they are frequently used to label various intracellular processes. In synthetic biology, fluorescent reporters are often used as the output of a genetic circuit, for example to signal the detection of a chemical.

As part of our iGEM project, we implemented a new fluorescent reporter, Dendra 2. In addition, we introduced a new coding sequence for superfolder GFP that is codon optimised for E.coli.

Dendra 2

Dendra 2 is a green fluorescent protein that can be irreversibly single-photon photoconverted into a red fluorescent protein.

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